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These histological studies also indicated that MG53 is absent from smooth muscle and vascular endothelial cells (Fig. 1f). Because of the difficulty of isolating primary cultured type I and type II alveolar epithelial cells from mice, we performed immunoblotting with cultured adenocarcinoma human type II alveolar epithelial cells (A549), human lung microvascular endothelial cells, human pulmonary artery endothelial cells and human umbilical vein endothelial cells.